Compound serum and hemin free medium for cultivation of Leishmania tarentolae: A recombinant protein expression system
نویسندگان
چکیده
منابع مشابه
Recombinant protein expression in Leishmania tarentolae.
A variety of recombinant protein expression systems have been developed for heterologous genes in both prokaryotic and eukaryotic systems such as bacteria, yeast, mammals, insects, transgenic animals, and plants. Recently Leishmania tarentolae, a trypanosomatid protozoan parasite of the white-spotted wall gecko (Tarentola annularis), has been suggested as candidate for heterologous genes expres...
متن کاملCharacterization of the growth behavior of Leishmania tarentolae: a new expression system for recombinant proteins.
Biotechnological production of recombinant proteins for human therapy requires a cultivation of the host organism in a nutrient medium free of animal substances. Therefore, various nutrient media for the new expression system Leishmania tarentolae were developed and examined according to their cultivation conditions as static suspension culture and agitated culture. Investigations resulted in t...
متن کاملThe production of recombinant human laminin-332 in a Leishmania tarentolae expression system.
Laminin (LM)-332 (alpha3beta3gamma2), a large heterotrimeric glycoprotein, is an essential component of epithelial basement membranes that promotes cell adhesion and migration. Here, we expressed human LM-332 using a novel protein expression system based on the trypanosomatid protozoan host Leishmania tarentolae. Plasmids containing cDNA encoding full-length beta3 and gamma2 subunits and trunca...
متن کاملTowards the Construction of Expressed Proteomes Using a Leishmania tarentolae Based Cell-Free Expression System
The adaptation of organisms to a parasitic life style is often accompanied by the emergence of novel biochemical pathways absent in free-living organisms. As a result, the genomes of specialized parasitic organisms often code for a large number (>50%) of proteins with no detectable homology or predictable function. Although understanding the biochemical properties of these proteins and their ro...
متن کاملLeishmania cell-free protein expression system.
Cell-free protein expression is an important tool for a rapid production, engineering and labeling of recombinant proteins. However the complex protocols for preparation of eukaryotic cell-free protein expression systems result in high manufacturing costs and limit their utility. Recently we reported a novel cell-free expression system based on the lysate of a fermentable protozoan Leishmania t...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2015
ISSN: 1684-5315
DOI: 10.5897/ajb2015.14654